Public sector provides various essential services in terms of promoting social equity and inclusion, protecting the public interest, regulating and supervising various aspects of society and economy, fostering economic development, and supporting democratic governance. Therefore, the motivation of the public sector servants indirectly contributes to the overall effectiveness of the public service sector and finally to the well-being and prosperity of the society. Considering this, in the public sector, extrinsic factors cannot be easily and quickly influenced, i.e., they are fixed. This paper can be of crucial importance when determining the intrinsic factors behind the public sector servants’ satisfaction with their jobs and ultimately their performance. Considering this, in developing economies, such as the economy of the Republic of North Macedonia, public sector servants exhibit poor performance. The main purpose of the research presented in this paper has been to investigate the motivational factors of the public sector servants at municipality level in the Republic of North Macedonia. In the research, primary data collected by using descriptive statistics and cross-tabulation have been used to determine whether gender-based differences exist in intrinsic motivation.